{"id":33,"date":"2017-01-05T20:00:27","date_gmt":"2017-01-05T20:00:27","guid":{"rendered":"http:\/\/wordpress.uchospitals.edu\/basu-lab\/?page_id=33"},"modified":"2017-03-23T00:48:52","modified_gmt":"2017-03-23T00:48:52","slug":"high-throughput-in-vivo-drug-screening","status":"publish","type":"page","link":"https:\/\/medpress.bsd.uchicago.edu\/basu-lab\/high-throughput-in-vivo-drug-screening\/","title":{"rendered":"High throughput Drug Screening in vivo"},"content":{"rendered":"
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High-throughput drug screening conducted using various technologies, such as robotic handling and micro-titer plates, have significantly advanced drug development. However, the costs and time associated with such technologies are exorbitant. Droplet microfluidics provides a popular lab-on-a-chip<\/em> technique where reagents may be combined in pico-liter volumes in a fast and controlled manner. We use such devices to generate water-in-oil emulsion droplets at high throughput (~5k-10k drops per second) that efficiently encapsulate cells in presence of drugs. Reducing the size of the reaction compartments to pico-liter volumes allow us to be parsimonious with reagents while the large number of droplets (~105<\/sup>-106<\/sup>) provides superior statistical resolution.<\/p>\n

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In this project, we use two sets of microfluidic devices to test the efficacy of cancer chemotherapy drugs on human cell lines where the drug concentrations are systematically varied. A fluorescent dye indicates the chemotherapy drug concentration (see figure to the left; drug concentration is proportional to the fluorescent intensity of the drop). The efficacy of the drugs is measured as functions of exposure time and drug concentration from the cell-fate of human lymphoblast cells (LCL) in each drop after co-incubation, using fluorescent live\/dead cell-fate reporter kit.<\/p>\n

After the drops are generated, we collect the drops off chip, incubate them for different time intervals, and then re-inject the drops into a second microfluidic chip where they are excited by a laser beam and the resulting fluorescent emission from the drug and cells (dead\/alive) are simultaneously detected using photo-multiplier tubes (PMT). The PMT voltage, indicative of fluorescent intensity, reports drug concentration, while the duration of the signal distinguishes a drug-laden drop from a live cell (bottom, left). Overall statistical information is aggregated onto a heat-map shown on bottom, right.<\/p>\n

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High-throughput drug screening conducted using various technologies, such as robotic handling and micro-titer plates, have significantly advanced drug development. However, the costs and time associated with such technologies are exorbitant. Droplet microfluidics provides a popular lab-on-a-chip technique where reagents may be combined in pico-liter volumes in a fast and controlled manner. We use such devices … <\/p>\n

Continue reading “High throughput Drug Screening in vivo”<\/span><\/a><\/p>\n","protected":false},"author":233,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-33","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/medpress.bsd.uchicago.edu\/basu-lab\/wp-json\/wp\/v2\/pages\/33","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/medpress.bsd.uchicago.edu\/basu-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/medpress.bsd.uchicago.edu\/basu-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/medpress.bsd.uchicago.edu\/basu-lab\/wp-json\/wp\/v2\/users\/233"}],"replies":[{"embeddable":true,"href":"https:\/\/medpress.bsd.uchicago.edu\/basu-lab\/wp-json\/wp\/v2\/comments?post=33"}],"version-history":[{"count":8,"href":"https:\/\/medpress.bsd.uchicago.edu\/basu-lab\/wp-json\/wp\/v2\/pages\/33\/revisions"}],"predecessor-version":[{"id":320,"href":"https:\/\/medpress.bsd.uchicago.edu\/basu-lab\/wp-json\/wp\/v2\/pages\/33\/revisions\/320"}],"wp:attachment":[{"href":"https:\/\/medpress.bsd.uchicago.edu\/basu-lab\/wp-json\/wp\/v2\/media?parent=33"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}